Addressing The Complexities And Challenges In Lentivirus Harvest Clarification

Gene therapy holds immense promise for treating a wide range of diseases by modifying a patient's own genetic material. Lentiviral vectors are a powerful tool for delivering this genetic code, but their production faces a critical hurdle: harvest clarification.

Traditional depth filtration,f the workhorse of bioprocessing, struggles with lentiviruses. These delicate, enveloped viruses tend to cling to filter surfaces, leading to significant losses during clarification. This not only reduces the final yield of the therapeutic product but also necessitates larger starting volumes, driving up costs. Additionally, depth filtration can be a time-consuming and labor-intensive process.

In this case study, we examine a promising alternative: single-use centrifugation. By gently separating cells and debris from the valuable lentiviral particles, this method offers several advantages. Download the full case study to learn more.